New development in the study of tumor-stromal interaction focused on extracellular miRNA via exosome.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: TOMA Hiroki 九州大学, 医学(系)研究科(研究院), 共同研究員 (80437780)
• Co-Investigator(Kenkyū-buntansha): TANAKA Masao 九州大学, 医学研究院, 教授 (30163570) ; TOMINAGA Yohei 九州大学, 医学研究院, 共同研究員 (90304823) ; OHUCHIDA Kenoki 九州大学, 大学病院, 助教 (20452708) ; MIYASAKA Yoshihiro 九州大学, 医学研究院, 助教 (40507795)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 膵癌 / miRNA / 癌間質相互作用

Outline of Final Research Achievements

We planed to identify key-miRNAs that correlate with metastasis. First, we checked the effect of miR-373 reported to correlate with metastasis of breast cancer and investigated that transfection of miR-373 induced MET progression in SUIT2 and KP2 cell lines. Next, we analyzed comprehensive miRNA expression of highly metastatic pancreas cancer cell line and normal pancreas cancer cell line. We identified several miRNAs highly expressed in one side. For example, miR-125b-5p, miR-614, miR-3178, miR-4324 and miR-4706 were up-regulated and miR-16-5p, miR-27b-3p, miR-192-5p and miR-194-5p were down-regulated in metastatic cell line. These miRNAs may affect cancer metastasis, because MiR-16, miR-27b, miR-192 and miR-125b were already reported to correlate with metastasis.

Free Research Field

医歯薬学