2014 Fiscal Year Final Research Report
Development of cancer treatment targeting morphogen and cancer stem cell
| Project/Area Number |
24592043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Hiroshi 川崎医科大学, 医学部, 講師 (60623048)
TSUTSUMI Kosuke 川崎医科大学, 医学部, 助教 (00467937)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 膵癌 / 癌微小環境 / 形態形成シグナル / Hedgehog signaling / Sonic Hedgehog / metformin / AMPK |
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| Outline of Final Research Achievements |
[Aim] The aim of this study is to explore the relationship between metformin and Hh signaling pathway, and to clarify the mechanism by which metformin prevents cancers. [Materials and methods] Expression of Shh was examined at protein and mRNA level with or without metformin by real-time PCR, immunoblotting and immunestaining. Phosphorylation of AMPK was detected by immunoblotting. [Results] Shh expression was decreased at protein and mRNA level by addition of metformin in BxPC3 cells. Phosphorylation of AMPK was increased by metformin and decreased by dorsomorphin. Interestingly, Shh expression was increased by dorsomorphin. This effect was further confirmed by siRNA of AMPK. [Conclusion] Metformin inhibits production of Shh in BxPC3 through phosphorylation of AMPK. Activation of AMPK might be one of the routes to link metformin and suppression of Shh.
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| Free Research Field |
消化器外科学
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