2014 Fiscal Year Final Research Report
The in vitro research of bacterial infection of prosthetic vascular grafts.
| Project/Area Number |
24592073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Yuki 東邦大学, 医学部, 助教 (40385729)
FUJII Takeshiro 東邦大学, 医学部, 准教授 (80287531)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 人工血管感染 / 緑膿菌 / 電子顕微鏡 |
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| Outline of Final Research Achievements |
Bacterial infection from outer surface of prosthetic vascular graft is a subject of our study. The research was done with the models of contaminated vascular graft to investigate the mechanism of bacterial invasion into prosthetic vascular graft from outer surface. Pseudomonas aeruginosa was selected as the model bacterium for its mobility and ability to thrive in physiological saline. Furthermore, a cross section of the vascular grafts were observed with scanning electron microscopy to confirm the bacterial invasion into vascular grafts. Recently, we perform the experiment to measure the amount of gene of protease from bacteria contacted with gelatin-coated Dacron vascular graft by RT-PCR method. Its pilot study is now ongoing.
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| Free Research Field |
心臓血管外科学
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