Bone healing with the adipose-derived regenerative cells in the large bone defect after total en bloc spondylectomy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: DEMURA Satoru 金沢大学, 大学病院, 講師 (00348228)
• Co-Investigator(Renkei-kenkyūsha): INOUE Kei 金沢大学, 大学病院, 医員 (00622862) ; HAYASHI Katsuhiro 金沢大学, 大学病院, 助教 (80507054) ; MURAKAMI Hideki 金沢大学, 医学系, 准教授 (70334779) ; TSUCHIYA Hiroyuki 金沢大学, 医学系, 教授 (40227434)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脊椎腫瘍 / 腫瘍脊椎骨全摘術 / 自家骨移植 / 自己脂肪由来幹細胞 / 骨癒合 / 脊柱再建 / 生物学的固定 / 骨欠損

Outline of Final Research Achievements

We investigated whether adipose derived regenerative cells (ADRCs) promote osteogenesis of the bone graft in total en bloc spondylectomy (TES) animal model. In control group, bone formation area was occupied by woven bone 2 weeks after surgery. In ADRCs group, lamellar bone was shown in a certain proportion 2 weeks after surgery, and the ratio of lamellar bone increased in both groups 4weeks after sugery. The Number of osteoblasts lined up on the osteoid surface tended to be large in ADRCs group. Under fluorescent light, there was few deposition of calcein in control group, in the meanwhile, there were defuse deposition which showed immature lamellar structure in ADRCs group 2 weeks after surgery. The orderly label line was shown that meant mature lamellar structure in both groups 4weeks after surgery. ADRCs used as materials of spinal reconstruction promoted osteogenesis of the bone graft in the early phase.

Free Research Field

医歯薬学