Bone regeneration using culture-expanded bone marrow cells-Efficacy of Runx2-activating drugs-

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya University
• Principal Investigator: HIROSHI Kitoh 名古屋大学, 医学(系)研究科(研究院), 准教授 (40291174)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Runx2 / ランソプラゾール / 骨組織再生 / 培養骨髄細胞

Outline of Final Research Achievements

We screened for drugs that activate the Runx2 promoter and identified that lansoprazole, a proton pump inhibitor, upregulated the Runx2 activity. Lansoprazole elevated expression levels of Runx2 mRNA and protein in osteoblast-lineage cells in a dose-dependent manner. Lansoprazole exerted an increase of ALP and Osterix activities in osteoblast-lineage cells. Lansoprazole accelerated the matrix calcium deposition in human bone marrow derived osteoprogenitors. Systemic administration of lansoprazole to a rat fracture model increased osteoblastic parameters and facilitated fracture healing. We propose that lansoprazole holds promise as a therapeutic agent for bone regeneration at fracture sites.

Free Research Field

整形外科学