DNA double strand break and fusion gene formation in tumorigenesis of sarcoma.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592250
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: National Hospital Organization, Kyushu Cancer Center
• Principal Investigator: TANAKA Kazuhiro 独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, 客員研究員 (10274458)
• Co-Investigator(Kenkyū-buntansha): ODA Shinya 独立行政法人国立病院機構九州がんセンター, 臨床研究センター, 腫瘍遺伝学研究室長 (40333372)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 肉腫 / 骨軟部腫瘍 / 染色体転座 / DNA修復

Outline of Final Research Achievements

Using DNA microarray approaches, expression of the genes functioning in DNA double strand break (DSB) repair was explored in cell lines or tissue specimens derived from Ewing sarcomas and the other human soft tissue neoplasms with disease-specific chromosomal translocations. Expression of several DSB repair genes was similarly induced in these sarcoma cells. Furthermore, expression and subcellular localization of the DSB repair proteins were observed using immunocytochemistry, the results revealing an enhanced nuclear foci-formation in some DSB repair factors, which strongly suggest that the induction of the genes does reflect functional responses to DSBs occurred in cells.

Free Research Field

整形外科学