Exosomal small RNA in the plasma of early pregnant women as a significant predictor of pregnancy-induced hypertension

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Hokkaido University
• Principal Investigator: FURUTA Itsuko 北海道大学, 医学(系)研究科(研究院), 助手 (70238682)
• Co-Investigator(Kenkyū-buntansha): YAMADA Takahiro 北海道大学, 大学院医学研究科, 特任講師 (20419948) ; KANEUCHI Masanori 長崎大学, 大学病院, 講師 (60333613)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: exosome / 妊娠高血圧症候群 / small RNA

Outline of Final Research Achievements

To determine whether plasma exosomal small RNA levels (exo-RNA) in early pregnancy can predict the onset of pregnancy-induced hypertension(PIH), we investigated exo-RNA in early pregnant women.Plasma samples were collected from singleton pregnant women (6-19 weeks of gestational age). Written informed consent was obtained from all participants of this study. Exosomal RNA extracted from plasma were analysed by Agilent 2100 Bioanalyzer.Three size of nucleotides (nt) area were identified in exo-RNA with Bioanalyzer electropherogram, and each peak areas were categorized as micro (10 to 40 nt), medium (41to 80 nt) and large (81 nt<) RNA.Exo- micro and medium RNA in women without PIH at 9-11 weeks of gestation were significantly higher than those at 6-8 weeks of gestation. Also longitudinal significant increase was observed in exo-RNA in women at 6-11 weeks of gestation.At 6-8 weeks of gestation, Exo- micro RNA in women with PIH was significantly higher than those without PIH (p-0.038).

Free Research Field

産科・周産期医学