Studies of the spermatogenic cell-spesific expression of the exogenous gene.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592465
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: University of Yamanashi
• Principal Investigator: SHODA Tomoko 山梨大学, 総合研究部, 助教 (50345716)
• Co-Investigator(Kenkyū-buntansha): HIRATA Shuji 山梨大学, 総合研究部, 教授 (00228785)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 遺伝子治療 / 遺伝子導入 / 生殖細胞

Outline of Final Research Achievements

Recently, the fertility treatment for the severe oligospermatozoa or azospermatozoa was improved by the intracytoplasmic sperm injection (ICSI). However, the abnormality of male spermatogenesis will be hereditary to their next stage. In this case, if the treatment to get the normal sperm with the gene transfer to the testis is possible, the abnormal gene will not be inherited to the next generation. For this purpose, we try to gene transfer to the mice testis in vivo to get the normal sperm. The combination of the plasmid cDNA injection into seminiferous tubules and the electroporation (EP) has become an efficient and convenient assay system for spermatogenetic specific gene expression during spermatogenesis of mice. In this study, we evaluated the possibility of this technique using the fluorescent protein as a marker. Using this technique, it would be a useful method as a gene therapy for the abnormal sperm.

Free Research Field

分子内分泌