Possible mechanism of remodeling of maternal spiral artery by trophoblast

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592470
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyoto University
• Principal Investigator: YUKIYASU Sato 京都大学, 医学(系)研究科(研究院), 非常勤講師 (00508236)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Hiroshi 金沢大学, 医学研究科, 教授 (30252456)
• Co-Investigator(Renkei-kenkyūsha): HORIE Akihito 京都大学, 医学研究科, 助教 (30535836)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 絨毛外トロホブラスト / 母体血管リモデリング / 血管内トロホブラスト

Outline of Final Research Achievements

Purpose of our research is to elucidate possible mechanism of maternal vascular remodeling by extravillous trophoblast (EVT) that takes place at human implantation sites. Here, we demonstrated that both CD9 and CD59 are highly and specifically expressed on EVT invading maternal vessels (eEVT). Accordingly, functional experiments were carried out using immotile EVT cells (Swan71) and choriocarcinoma cell line (BeWo).
As for CD9, knock down of this molecule inhibited lumen formation in Swan71, suggesting that CD9 is involved in the replacement of maternal endothelium by eEVT. As for CD59, we found that Swan71 but not BeWo expresses this molecule, and further experiments are currently in progress.

Free Research Field

生殖医学