Basic study for new ovulation induction using hypothalamic neuropeptide

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokushima
• Principal Investigator: MATSUZAKI Toshiya 徳島大学, ヘルスバイオサイエンス研究部, 准教授 (70294692)
• Co-Investigator(Kenkyū-buntansha): IRAHARA Minoru 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (20160070)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ペプチド / 排卵誘発 / 排卵障害 / ゴナドトロピン / GnRH / 多嚢胞性卵巣症候群 / レトロゾール

Outline of Final Research Achievements

We studied the effect of choronic treatment of senktide for recovery of estrous cycle in rat ovulatory disorder model in order to develop new ovulation induction method that has low incidence of multiple pregnancy. We induced rat ovulatory disorder model using letrozol. Letrozol releasing pellets (0.4 mg/day) were placed subcutaneously on the back of 3-week-old female Wistar rats. The estrous cycle was estimated daily by vaginal smear after vaginal opening. These rats did not show estrous cycle and had no corpus lutein and possessed more follicles, showed higher leptin, lower estradiol, higher LH serum levels, higher expression of Kisspeptin mRNA in the posterior hypothalamic area than rats of control group. We placed osmotic minipump that released senktide (0.2 mg/day) for 3 weeks subcutaneously on the back of 8-week-old female rat ovulatory disorder model. Based on assessment of daily vaginal smear, senktide did not recover estrous cycle in the rat ovulatory disorder model.

Free Research Field

医歯薬学