Population shift of the nasopharyngeal microbiota of infants with acute otitis media.

2015 Fiscal Year Final Research Report

• Project/Area Number: 24592558
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Wakayama Medical University
• Principal Investigator: Ogami Masashi 和歌山県立医科大学, 医学部, 博士研究員 (90423946)
• Co-Investigator(Kenkyū-buntansha): Togawa Akihisa 和歌山県立医科大学, 医学部, 講師 (70305762) ; Hotomi Muneki 和歌山県立医科大学, 医学部, 准教授 (90336892)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 鼻咽腔細菌叢 / 群集解析 / 主成分分析

Outline of Final Research Achievements

We conducted a culture-independent molecular analysis, i.e., the population analysis, on 22 nasal washes of infants with AOM and 9 nasal washes of healthy infants.
The principal component analysis revealed that two commensal bacteria, Dolosigranulum-pigrum and Corynebacterium-propinquum, were major microbiota in nasal washes of healthy infants. On the other hand, nasopharyngeal microbiota of infants with AOM were characterized by 3 major pathogenic bacteria, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae.
Population analysis with PCA and UPGMA of microbiota in infants with AOM revealed the clear and significant difference from healthy infants. Diverse pathogenic microbiota of nasal washes in healthy and AOM infants by the concurrent analysis indicated dynamic transition of microbiota from harmless commensals in healthy state to pathogenic microbiota in the nasopharynx in AOM.

Free Research Field

感染症