Identification of tumor-suppressive microRNAs regulating metastatic pathways in HNSCC

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592590
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Chiba University
• Principal Investigator: SEKI Naohiko 千葉大学, 医学(系)研究科(研究院), 准教授 (50345013)
• Co-Investigator(Kenkyū-buntansha): HANAZAWA Toyoyuki 千葉大学, 大学院医学研究院, 准教授 (90272327)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: マイクロRNA / 下咽頭癌 / 癌抑制遺伝子 / 発現プロファイル

Outline of Final Research Achievements

In this study, we have developed miRNA expression signatures of hypopharyngeal squamous cell carcinoma (HSCC) using clinical specimens. Using those data, we have investigated the specific roles of miRNAs in HSCC metastasis by examining the differentially expressed miRNAs. Our hypopharyngeal squamous cell carcinoma (HSCC) microRNA signature showed that miR-451a is significantly downregulated in cancer tissues, suggesting that miR-451a might act as a tumor suppressor.
Restoration of miR-451a in cancer cell lines revealed that this miRNA significantly inhibited cancer cell migration and invasion. Our data demonstrated that the gene coding for endothelial and smooth muscle cell-derived neuropilin-like molecule (ESDN/DCBLD2) was a direct target of miR-451a regulation.

Free Research Field

ゲノム医学