Explication of stem cells formation mechanism using fibroblasts spheres

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592719
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: HAYASHI RUKA 慶應義塾大学, 医学部, 助教 (50445392)
• Co-Investigator(Kenkyū-buntansha): TORIUMI Masahiro 帝京大学, 医学部, 助教 (20528210) ; KISHI Kazuo 慶應義塾大学, 医学部, 教授 (40224919)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞凝集塊 / 非接着培養 / 間葉系細胞 / 線維芽細胞 / 毛包誘導

Outline of Final Research Achievements

Mesenchymal cells were extracted and cultured from skin of newborn C57BL/6J mice, and each cell spheres were made using serum-free medium on non-adhesive culture dishes. Spheres were viable for the comparative long term of the months unit in spite of the non-adhesive condition, which suggest the possibility that the spheres were in so-called hibernation state. In genetic and immunohistological examination, the Wnt related markers which supposed to concerned with hair follicle regeneration and hair cycle, and also the undifferentiated markers such as Sox-2, Oct-4, CD133, cxcr4 were seen to relatively upregulated after forming the cell spheres. But the distribution of the markers was not equal in cell spheres, it is necessary to examine whether it is the result of character change in some specific cells or the result of the cell choice.

Free Research Field

形成外科学