2014 Fiscal Year Final Research Report
Experimental study of induction of cytokines through skin and ulcer base using electroporation
| Project/Area Number |
24592724
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAKUDO Natsuko 関西医科大学, 医学部, 助教 (00509490)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | PRP / cytokine / electroporation / regenerative medicine / skin / skin ulcer / 電圧 / パルス |
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| Outline of Final Research Achievements |
Skin and basis of skin ulcer have protective function not to permeate through them for medications and materials over 20kD molecules in the size. It is aimed to treat skin wounds and aging skin broadly, if medications and materials with larger molecule can be penetrated. In this time, how to penetrate 20-100kD growth factors minimally invasively trough the skin and the base of the skin ulcer using electroporation (EP method) is studied.
Any invasions in EP method, and permeating bFGF and PRP, which contains various growth factors, were investigated invasions. As comparing in voltages, in 80V light skin stimulation was observed only during induction. No skin and soft tissue damages remained. Cytokines penetrating the skin surface was confirmed, however, more detailed mechanisms will be investigated in the future.
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| Free Research Field |
形成外科学
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