2014 Fiscal Year Final Research Report
Relationship between development of Hertwig's epithelial root sheath and molecular mechanism that controls cell migration.
| Project/Area Number |
24592776
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
CHOUSA Naoyuki 岩手医科大学, 歯学部, 助教 (80326694)
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| Co-Investigator(Renkei-kenkyūsha) |
HARADA Hidemitsu 岩手医科大学, 歯学部, 教授 (70271210)
OTSU Keishi 岩手医科大学, 歯学部, 助教 (60509066)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 臼歯歯根形成 / ヘルトビッヒ上皮鞘 / 細胞遊走 / Rho signaling / アクチン / タモキシフェン誘導型ドミナントネガティブマウス / 器官培養 / HERS01a細胞株 |
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| Outline of Final Research Achievements |
We examined to clarify the role of Rho signaling (RS) for development of Hertwig’s epithelial root sheath (HERS) with implementation of gain/loss of function examinations using K14cre/tamoxifen-induced RS dominant negative mice, original organ culture for observation of postnatal tooth development, and cell culture of HERS-derived cell line, HERS01a. RS regulated cell morphology such as cell processes, and cell migration though modulation of actin localization in cytoplasm, and cell proliferation in HERS cells and HERS formation. Activation of RS induced long bilayered HERS on root dentin matrix without disintegration of epithelial sheet. Interestingly, this phenomenon was similar to effects induced by inhibitor of TGF-β. RS is closely related to formation and maintenance of bilayered HERS sheet and regulation of disintegration in HERS during mouse molar root development. Our studies revealed that RS is one of important mechanisms in regulation of root development.
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| Free Research Field |
口腔組織・発生学
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