Regulations of bone metabolism by bovine- and neutrophil-derived lactoferrin

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592786
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: NINOMIYA Tadashi 松本歯科大学, 総合歯科医学研究所, 講師 (00360222)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Hiroaki 松本歯科大学, 歯学部, 教授 (50227930) ; HIRAGA Toru 松本歯科大学, 歯学部, 准教授 (70322170) ; KOIDE Masanori 松本歯科大学, 総合歯科医学研究所, 講師 (10367617)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ラクトフェリン / 骨芽細胞 / 破骨細胞 / 細胞分化

Outline of Final Research Achievements

To elucidate the roles of lactoferrin for the bone metabolism, we examined the effects of bovine- (bLF) and neutrophil-derived lactoferrin (nLF) on osteoblast and osteoclast differentiation. In vitro studies using mesenchymal stromal cells (MSCs) showed that bLF and nLF promoted the mRNA expressions of osteoblast markers and increased a number of ALPase-positive cells. On the other hand, formation of TRAP-positive cell from bone marrow macrophages (BMMφ) and the mRNA expressions of osteoclast markers in BMMφ were inhibited by these LF. In addition, neutrophil-cultured conditioned medium accelerated osteoblast differentiation of MSCs and inhibited osteoclast differentiation of BMMφ. Lipoprotein receptor related protein 1 as a receptor of lactoferrin expressed in BMMφ with the stimulation of RANKL and MSCs.

Free Research Field

口腔解剖学