Survival of salivary pleomorphic adenoma cells under the hypoxia-responsive extracellular matrix biosynthesis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592829
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Niigata University
• Principal Investigator: MARUYAMA Satoshi 新潟大学, 医歯学総合病院, 講師 (30397161)
• Co-Investigator(Kenkyū-buntansha): ABE Tatsuya 新潟大学, 医歯学総合病院, レジデント (70634856) ; YAMAZAKI Manabu 新潟大学, 医歯学系, 助教 (10547516) ; CHENG Jun 新潟大学, 医歯学系, 准教授 (40207460) ; SAKU Takashi 新潟大学, 医歯学系, 教授 (40145264)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 実験腫瘍学 / 低酸素 / 唾液腺多形性腺腫

Outline of Final Research Achievements

Salivary pleomorphic adenoma is histopathologically characterized by its colorful stroma with myxoid, chondroid, and hyaline appearances, which is realized by its enhanced biosynthesis of extracellular matrix (ECM) molecules as well as by poor vascularity. Thus, pleomorphic adenoma cells embedded in such stromata are supposed to be able to survive in hypoxic conditions. To understand the hypoxia-dependent manner of cellular proliferation in this particular tumor, we analyzed function of perlecan and fibronectin, which are the most abundant ECM molecules, in cell proliferation in hypoxic-conditioned pleomorphic adenoma cells. Hypoxic conditions induce pleomorphic adenoma cells to produce perlecan and fibronectin, which is maintained by high HIF-1α protein levels, which is further realized by perlecan and fibronectin-rich circumstance of the stroma. The results indicate that pleomorphic adenoma cells have to go round in hypoxic circles to survive.

Free Research Field

医歯薬学