2014 Fiscal Year Final Research Report
A study on the mechanisms of tissue wound healing and regeneration of dental pulp
| Project/Area Number |
24592863
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIBA Nagako 新潟大学, 医歯学総合病院, 講師 (10323974)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 象牙質・歯髄複合体 / 歯髄組織幹細胞 / 直接覆髄 / 組織培養 / 象牙芽細胞 |
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| Outline of Final Research Achievements |
The aim of this study was to elucidate cellular events during pulp tissue wound healing and reparative dentin formation after direct pulp capping. M2 macrophage-associated molecule-expressing cells transiently accumulated beneath the exposure site. The deposition of osteopontin and dentin matrix protein 1 (DMP1) at exposed pulp sites preceded the appearance of nestin-immunoreactive cells, active cell proliferation and new matrix formation. These suggest that M2 macrophages participate in the initial phases of the healing and that osteopontin and DMP1 act as a trigger of pulp repair. In addition, numerous α-smooth muscle actin (SMA)-positive cells emerged at the wound margin, and the initially formed mineralized barrier was lined with α-SMA-positive cells similar in appearance to reparative odontoblasts, some of which co-expressed nestin. Fibrillin-1 degradation and down-regulation might be implicated in the differentiation of α-SMA-positive cells in this process.
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| Free Research Field |
歯科保存学
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