Risk assessment of prosthodontic treatment by peripheral benzodiazepine receptor gene polymorphism

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592926
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: MASAKI Chihiro 九州歯科大学, 歯学部, 助教 (60397940)
• Co-Investigator(Kenkyū-buntansha): HOSOKAWA Ryuji 九州歯科大学, 歯学部, 教授 (60211546) ; NAKAMOTO Tetusji 九州歯科大学, 歯学部, 准教授 (30514989) ; KONDO Yusuke 九州歯科大学, 歯学部, 助教 (00611287)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 睡眠時ブラキシズム / 遺伝子多型

Outline of Final Research Achievements

Peripheral-type benzodiazepine receptor (PBR) gene polymorphism is associated with stress sensitivity, but it is not yet clear whether polymorphisms in this gene have any influence on psychological stress. The present study was performed to examine the relationship between sleep bruxism and PBR gene polymorphism. The PBR gene was amplified using phi29 DNA polymerase after collection of DNA from oral mucosa cells. We also measured masseter muscle activity during sleep using portable electromyography to evaluate sleep bruxism. Furthermore, stimulated whole-saliva samples were collected for quantification of salivary chromogranin A (CgA) level. Thirty-two subjects (68.1%) had the G/G genotype, 13 (27.6%) had G/A, and 2 had A/A. The number of bruxism events was significantly higher in the G/A than the G/G group. Changes in salivary CgA levels were significantly lower in the G/A than G/G group. PBR gene polymorphism may influence psychological stress management through bruxism during sleep.

Free Research Field

医歯薬学