Proteome analysis and energy dispersive X-ray analysis in masticatory muscle tendon-aponeurosis hyperplasia

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Saitama Medical University
• Principal Investigator: YODA Tetsuya 埼玉医科大学, 医学部, 教授 (60242210)
• Co-Investigator(Kenkyū-buntansha): AKITA Masumi 埼玉医科大学, 医学部, 教授 (60105905) ; SAKAMOTO Yasushi 埼玉医科大学, 医学部, 教授 (80178582) ; SATO Tsuyoshi 埼玉医科大学, 医学部, 准教授 (60406494) ; SAKATA Yasuaki 埼玉医科大学, 医学部, 准教授 (50322425)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 咀嚼筋腱・腱膜過形成症 / プロテオーム解析 / 元素分析 / ケイ素 / 石灰化 / 腱

Outline of Final Research Achievements

Masticatory muscle tendon-aponeurosis hyperplasia (MMTAH) is a new disease entity associated with limited mouth opening. In this study, we analyzed the microstructural characteristics of muscles and tendons in MMTAH by electron microscopy and energy dispersive X-ray analysis to determine the elemental composition. Histological analysis was performed to detect the calcification. Transmission electron microscopy and scanning electron microscopy were conducted to clarify the microstructural characteristics of muscles and tendons. Energy dispersive X-ray microanalysis was performed to identify the distribution of elements. Mineralized nodules were observed in tendon tissues of MMTAH as compared with facial deformity. Electron microscopy revealed that the muscle and tendon tissues in MMTAH showed degenerative changes and distinctive histological findings as compared with tissues in facial deformity. We found that Ca, P, and Si were detected only in MMTAH.

Free Research Field

口腔外科