Affibody probes for near-infrared fluorescence imaging of cancer cells in sentinel lymph nodes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: TSUCHIMOCHI Makoto 日本歯科大学, 新潟生命歯学部, 教授 (20095186)
• Co-Investigator(Renkei-kenkyūsha): HAYAMA Kazuhide 日本歯科大学, 新潟生命歯学部, 准教授 (60120713) ; TSUBOKAWA Norio 新潟大学, 教育研究院・工学フェロー, 名誉教授 (20018675) ; YOSHIE Sumio 日本歯科大学, 新潟生命歯学部, 教授 (30095278) ; KAMETA Ayako 日本歯科大学, 新潟生命歯学部, 講師 (00328866)
• Research Collaborator: FUJII Hirofumi 国立がん研究センター, 先端医療開発センター・機能診断開発分野, 分野長 (80218982) ; KAWASE Tomoyuki 新潟大学, 歯学部・歯科基礎移植再生学, 准教授 (90191999) ; OKADA Yasuo 日本歯科大学, 新潟生命歯学部, 教授 (40267266) ; YAMAGUCHI Haruka (TAKEZAWA Haruka) 日本歯科大学, 新潟生命歯学部, 助教 (00756942)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: センチネルリンパ節 / バイオプシー / リンパ節転移 / シンチグラフィ / ICG / 近赤外蛍光 / 蛍光イメージング

Outline of Final Research Achievements

Sentinel lymph node biopsies have been used to determine the nodal stage in clinically node-negative cancers, such as breast cancer and skin melanoma. However, this procedure can not directly confirm the existence of metastatic cells within the sentinel lymph node. In this study, we aimed to investigate the specificity of a near-infrared HER2-targeting Affibody in imaging metastatic tumor cells within the sentinel lymph node. Fluorescent signals of the Affibody probes were observed in HER2-expressing breast cancer cells. Our data suggest that the ICG-fluorescent Affibody probes may enable direct visualization of HER2-overexpressing cancer cells in sentinel lymph nodes. Additional animal studies are required to elucidate the value of this approach in sentinel lymph node biopsy.

Free Research Field

外科系歯学/病態科学系歯学(含放射線系歯学)