Development of clinicopathological diagnosis of lymphangiogenesis and lymphatic invasion as a prognostic factor in tongue squamous cell carcinoma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Azabu University (2014); The Nippon Dental University (2012-2013)
• Principal Investigator: SHIMAZU YOSHIHITO 麻布大学, その他部局等, 准教授 (10297947)
• Co-Investigator(Kenkyū-buntansha): YAGISHITA Hisao 日本歯科大学, 歯学部, 准教授 (50256989) ; SATO Kaori 日本歯科大学, 歯学部, 講師 (90287772) ; TAYA Yuji 日本歯科大学, 歯学部, 准教授 (30197587) ; AOBA Takaaki 日本歯科大学, 歯学部, 教授 (30028807)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 歯学 / 病理学 / 舌癌 / リンパ管新生 / リンパ管浸潤 / 転移 / 病理診断 / 3次元形態計測

Outline of Final Research Achievements

The present study aimed at　developing clinicopathological diagnosis of lymphangiogenesis and lymphatic invasion as a prognostic factor in tongue squamous cell carcinoma. During the approved 3-year period, 3D reconstruction was achieved using 100 serial histological sections that were prepared from the invasion front of tongues squamous cell carcinomas. Cancer parenchyma and stroma were characterized by stepwise immunohistochemistry in combination with antibodies (cytokeratin, Ki67, CD31, D2-40, S100A4). 3D inspection in combination of epithelial and mesenchymal markers provided direct evidence for loco-regional invasion patterns, divergent intra- and peritumor microvessel densities, high frequencies of spatial association between carcinoma cells and blood/lymph endothelial cells. The results support the wide potential usage and advantage of histology-based 3D reconstruction in understanding of cancer biology and pathological diagnosis of tumor aggressiveness and malignancy.

Free Research Field

医歯薬学