Effect of osteocyte in TNF-a-induced osteoclast formation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593075
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: KITAURA Hideki 東北大学, 歯学研究科(研究院), 准教授 (60295087)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Teruko 東北大学, 大学院歯学研究科, 教授 (00127250) ; TAKESITA Nobuo 東北大学, 大学院歯学研究科, 助教 (50431515) ; SEIRYU Masahiro 東北大学, 大学院歯学研究科, 助教 (80510023)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 破骨細胞 / 骨細胞 / TNF-α

Outline of Final Research Achievements

We purified osteocytes from mouse carvariae by using collagenase and EDTA after removing soft tissues. We analyzed the purification of osteocyte by quantified expression level of DMP-1 with real-time PCR. We found that fraction 5 maximally included osteocytes. We cultured the cells in fraction 5 as osteocyte with TNF-α in α-MEM. Total RNA was isolated from adherent cells. We quantified expression level of osteoclast related genes. We found that expression of RANKL was increased in osteocytes cultured with TNF-α.

Free Research Field

矯正歯科