2014 Fiscal Year Final Research Report
Nobel periodontal regenerative therapy using paracrine factors from stem cells
Project/Area Number |
24593117
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Periodontal dentistry
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
IWASAKI Kengo 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (40401351)
|
Co-Investigator(Kenkyū-buntansha) |
KOMAKI Motohiro 東京医科歯科大学, 医歯(薬)学総合研究科, 寄付講座准教授 (30401368)
MORITA Ikuo 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60100129)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Keywords | 歯周病 / 再生 / 幹細胞 |
Outline of Final Research Achievements |
Periodontitis is chronic inflammatory disease, which is characterized by the destruction of the tooth supporting tissues. In this study, we investigated our hypothesis that transplantation of paracrine factors from periodontal ligament stem cells (PDLSC) could induce the regeneration of lost periodontal tissues. We transplanted 3 different paracrine factor preparations (Control-CM, PDLSC-CM and Fibroblast-CM) into surgically created periodontal defects in rats. After 4 weeks of transplantation, PDLSC-CM demonstrated significant new bone formation under micro-CT observations compared with other 2 groups. It was also revealed that PDLSC-CM contained various growth factors, cytokines and anigiogenic factors in protein array analysis. Moreover, we found that gene expression of TNF-alpha was reduced by the PDLSC-CM transplantation in regenerating rat periodontal tissue, suggesting the relationships between reduction of inflammation and tissue regeneration.
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Free Research Field |
再生
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