Molecular basis of phenotype alteration between planktonic and biofilm in Porphyromonas gingivalis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24593150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: KUBONIWA Masae 大阪大学, 歯学部附属病院, 講師 (00303983)
• Co-Investigator(Kenkyū-buntansha): FURUTA Nobumichi 大阪大学, 歯学研究科(研究院), 助教 (50452446) ; OJIMA Miki 大阪大学, 歯学研究科(研究院), 助教 (20263303) ; HASHINO Ei 大阪大学, 歯学部附属病院, 特任助教 (90614553)
• Co-Investigator(Renkei-kenkyūsha): AMANO Atsuo (50193024)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオフイルム / 歯周病原性菌 / Porphyromonas gingivalis / パラアミノ安息香酸

Outline of Final Research Achievements

S. gordonii cbe (chorismate binding enzyme: pABA syntase) deficient mutant developed short and flat biofilms, and failed to support P. gingivalis accumulation. In the low concentration of pABA (0.05 and 0.1 mg/ml), P. gingivalis levels increased although the biofilms retained the low and flat morphology. In contrast, 5 mg/ml of pABA drastically changed the microstructure of the mixed biofilms with prominent peaks and channels appearing, and this phenomenon was observed in both WT-P. gingivalis and DCBE-P. gingivalis. Incubation of P. gingivalis with pABA resulted in elevated (p<0.01) transcription of genes encoding both short and long fimbriae (mfa1 and fimA) and LuxS, which are required for the initial accumulation of P. gingivalis on streptococcal substrates.
Conclusion: The results suggest that Cbe of S. gordonii is essential for maturation of S. gordonii-P. gingivalis communities, and pABA is involved in communication with P. gingivalis.

Free Research Field

予防歯科学