Cancer prevention effect of calorie restriction on radiation-induced hepatocellular carcinoma in B63F1 mouse model

2014 Fiscal Year Final Research Report

• Project/Area Number: 24600030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 子ども学(子ども環境学)
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: SHANG Yi 独立行政法人放射線医学総合研究所, 福島復興支援本部, 研究員 (50533189)
• Research Collaborator: DAINO Kazuhiro 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 主任研究員 (90543299) ; MORIOKA Takamitsu 独立行政法人放射線医学総合研究所, 放射線防護研究センター, 主任研究員 (70253961) ; KAKINUMA Shizuko 独立行政法人放射線医学総合研究所, 放射線防護研究センター, チームリーダー (20392219)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: カロリー制限

Outline of Final Research Achievements

Children are especially sensitive to radiation, limiting their cancer risk is of great public concern. Earlier we reported in mouse model exposed to childhood radiation, adult-onset CR effectively suppressed liver tumor development. In this study, mutation frequencies of hepatocytes using gpt delta transgenic mouse were analyzed to investigate if CR contributes to decrease mutation accumulation. Hepatocellular carcinoma (HCC) samples were investigated for loss of heterozygosity (LOH), genomic copy number aberration and gene expression profiling. Even irradiated hepatocytes showed higher mutation frequency, there was no significant difference detected between standard and CR groups. CR groups showed lower frequencies of LOH and genomic copy number aberration. Differences in gene expression between standard and CR were also observed. These results suggested that CR might be related to the conservation of genomic stability, contributing to the suppression of HCC development.

Free Research Field

放射線発がん