Establishment of pluripotent stem cells with low tumorigenesity by use of genomic imprinting

2014 Fiscal Year Final Research Report

• Project/Area Number: 24613002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: epigenetics
• Research Institution: Gunma University
• Principal Investigator: HORII Takuro 群馬大学, 生体調節研究所, 助教 (00361387)
• Co-Investigator(Kenkyū-buntansha): HATADA Izuho 群馬大学, 生体調節研究所, 教授 (50212147)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 単為発生 / ゲノムインプリント / 腫瘍形成 / テラトーマ / 移植医療

Outline of Final Research Achievements

The objective of this study is how reduce tumor formation after transplantation of ES/iPS-derived tissues. Our study has shown that parthenogenetic ES (PgES) cells are more suitable than normal diploid ES cells in the point of tumorigenesis after transplantation. Detailed analyses of DNA methylation and gene expression clarified that Snrpn gene causes tumor formation. Profiling of DNA methylation and gene expression before transplantation of ES/iPS-derived tissues will bring more safety transplantation therapy.

Free Research Field

発生工学