Study on nucleosomes and chromatin-demodifying enzymes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24613007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: epigenetics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: UMEHARA Takashi 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (20415095)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 遺伝子発現 / エピジェネティクス / エピゲノム / クロマチン / 染色体 / ヒストン / ヌクレオソーム / 構造解析

Outline of Final Research Achievements

Purpose of this study is to establish biochemical assay systems for understanding of regulatory mechanisms of lysine-methylated nucleosome core particles through the histone demethylase family of LSD1/LSD2 (Lysine-specific demethylase-1/2) which is assumed in the onsets of intractable cancers and/or lifestyle-related diseases. We achieved cell-free protein production methodology to synthesize full-length histone H3 protein containing a site-specific mono-methyl-lysine, which is the demethylation substrates for LSD1/LSD2 in the context of chromatin, through the combination of site-specific incorporation of Boc-protected mono-methyl-lysine into a protein during translation and chemical removal of the Boc group.

Free Research Field

エピジェネティクス