2014 Fiscal Year Final Research Report
Characterization of neuronal regenerating factors secreted from rat bone marrow stromal cells
| Project/Area Number |
24615007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Aino University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IDE Chizuka 藍野大学, 医療保健学部・再生医療研究所, 教授 (70010080)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 神経再生 / 骨髄間質細胞 / 脊髄損傷 |
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| Outline of Final Research Achievements |
There are no effective therapies for spinal cord injury (SCI). We have reported that transplantation of bone marrow stromal cells (BMSCs) has beneficial effects on SCI. In the present study, we analyzed trophic factors secreted from BMSCs. At first, we improved the neural bioassay of neurite outgrowth using cultured hippocampal neurons of newborn rats. Next, we collected serum-free conditioned medium (CM) of rat BMSCs. We purified the effective factors on SCI from the CM by combination of liquid chromatographies and identified them using mass spectrometry systems.
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| Free Research Field |
生化学、神経再生
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