Novel in vivo imaging with the real time tracking system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24621001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 睡眠科学
• Research Institution: Hokkaido University
• Principal Investigator: HAMADA Toshiyuki 北海道大学, 医学(系)研究科(研究院), 特任准教授 (20360208)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 時計遺伝子 / 行動リズム / 光バイオイメージング / ルシフェラーゼ / ルシフェリン / 体内時計

Outline of Final Research Achievements

In this study, I developed a new system recording the gene expression in multiple areas of the freely moving mice with analyzing the body temperature and sleep-awake rhythm. In addition, new program to analyze the sleep-awake by tracing technique of the target area in freely moving mice was added into the system. Using this system, I succeeded in visualizing the internal desynchronization in freely moving mice. There were two type of tissues to express the phase shifts of clcok gene expression. One showed rapid phase shift with the same speed of that of locomotor activity rhythm, the others showed slow phase shifts. Present system is a powerful system for analyzing many areas of biomedical research, enabling simultaneous long term tracking of behavior and gene expression in multiple tissues.

Free Research Field

時間生物学