2013 Fiscal Year Final Research Report
Origin and fate of earliest generated cortical neurons
| Project/Area Number |
24650166
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka University |
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Principal Investigator |
MURAKAMI Fujio 大阪大学, 生命機能研究科, 教授 (20089882)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 大脳皮質 / 発生様式 / 興奮性神経細胞 / 子宮内電気穿孔 |
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| Research Abstract |
Neuroepithelial cells (NEs) in mouse cortex are thought to initially self renew and then generate basal progenitor cells (BP). Neurons are then generated from either of them by symmetric or asymmetric divisions. This idea is based on observations of mouse embryos at around E12.5 and how neurons are generated at earlier stages remains largely unknown.
Here we performed in utero electoroporation at E10.5 and observed progeny of labeled NEs in mature brain. We found that subplate cells, cortical excitatory neurons all all layers as well as astrocyes were labelled. This finding indicates that NEs generate subplate cells concomitantly with their self renewal. It also implies that these types of cells have common progenitor origins.
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