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2013 Fiscal Year Final Research Report

Development of a novel lentiviral vector system for the research of specific neural circuit labeling through retrograde axonal transport.

Research Project

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Project/Area Number 24650169
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Neuroscience in general
Research InstitutionFukushima Medical University

Principal Investigator

KATO Shigeki  福島県立医科大学, 医学部, 講師 (90443879)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords逆行性レンチウイルスベクター / エンベロープ / 神経回路
Research Abstract

We produced various types of fusion glycoproteins, in which the junction between the RVG and VSVG glycoprotein segments diverged in the membrane-proximal region of RVG, and generated HIV-1-based lentiviral vectors pseudotyped with these fusion glycoproteins. We then tested the efficiency of the pseudotyped vectors for the in vivo gene transfer through retrograde transport, comparing that of the NeuRet vector with FuG-C. We found a novel type of fusion glycoprotein, termed type E (FuG-E), that showed improved efficiency of retrograde gene transfer while retaining the property of neuron-specific transduction. This NeuRet vector with FuG-E will provide a powerful tool for genetic treatment of neurological and neurodegenerative diseases and for the study of neural circuit mechanisms underlying various brain functions.

  • Research Products

    (9 results)

All 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (4 results) Book (1 results)

  • [Journal Article] Improved transduction efficiency of a lentiviral vector for neuron-specific retrograde gene transfer by optimizing the junction of fusion envelope glycoprotein2014

    • Author(s)
      Kato, S., Kobayashi, K., and Kobayashi, K.
    • Journal Title

      J Neurosci. Methods

      Volume: 227 Pages: 151-158

    • Peer Reviewed
  • [Journal Article] Viral vector-mediated selective and reversible blockade of the pathway for visual orienting in mice2013

    • Author(s)
      Sooksawate, T., Isa, K., Matsui, R., Kato, S., Kinoshita, M., Kobayashi, K., Watanabe, D., Kobayashi, K., and Isa, T.
    • Journal Title

      Front. Neural Circuits

      Volume: 7 Pages: 162

    • Peer Reviewed
  • [Journal Article] Highly efficient retrograde gene transfer into motor neurons by a lentiviral vector pseudotyped with fusion glycoprotein2013

    • Author(s)
      Hirano, M., Kato, S., Kobayashi, K., Okada, T., Yaginuma, H., and Kobayashi, K.
    • Journal Title

      PLoS ONE

      Volume: 8(9) Pages: e75896

    • Peer Reviewed
  • [Journal Article] Elucidating information processing in primate basal ganglia circuitry : a novel technique for pathway-selective ablation mediated by immunotoxin2013

    • Author(s)
      Takada, M., Inoue, K., Koketsu, D., Kato, S., Kobayashi, K., and Nambu, A.
    • Journal Title

      Front. Neural Circuits

      Volume: 7 Pages: 140

    • Peer Reviewed
  • [Presentation] 高頻度逆行性ベクターによる大脳ー小脳ー基底核ネットワークへの遺伝子導入2014

    • Author(s)
      加藤成樹, 菅原正晃, 小林和人
    • Organizer
      平成25年度領域ミーティング(CREST)
    • Place of Presentation
      東京・JST東京本部別館1階ホール
    • Year and Date
      2014-03-02
  • [Presentation] 刺激弁別学習における2種類の主要な視床線条体路の行動生理学的役割2013

    • Author(s)
      加藤成樹, 深堀良二
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸・神戸ポートアイランド
    • Year and Date
      2013-12-05
  • [Presentation] Functional control of specific neural pathways in basal ganglia circuit by HiRet2013

    • Author(s)
      Shigeki KATO, Ryoji FUKABORI, Kenta Kobayashi, Hiromu Yawo, Yoshikazu Isomura and Kazuto KOBAYASHI
    • Organizer
      Neuroscience 2013
    • Place of Presentation
      SanDiego, CA, USA・San Diego Convention Center
    • Year and Date
      2013-11-09
  • [Presentation] Comparative analysis for behavioral roles of the central lateral and parafascicular nuclear groups projecting to the dorsolateral striatum2013

    • Author(s)
      Shigeki KATO, Ryoji FUKABORI and Kazuto KOBAYASHI
    • Organizer
      第36回日本神経科学大会
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2013-06-21
  • [Book] Gene Therapy (Francisco Martin Molina, ed)2013

    • Author(s)
      Kato, S., Kobayashi, K., Inoue, K., Takada, M., and Kobayashi, K. Croatia
    • Total Pages
      389-398
    • Publisher
      In Tech

URL: 

Published: 2015-06-25  

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