Construction of systematic tools forgenome analysesto determinegenes responsible for various disease model in rats

2012 Fiscal Year Final Research Report

• Project/Area Number: 24650238
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: The University of Tokushima
• Principal Investigator: IMOTO Issei (TACHIBANA Issei) 徳島大学, 大学院・へルスバイオサイエンス研究部, 教授 (30258610)
• Co-Investigator(Renkei-kenkyūsha): TAJIMA Atsushi 徳島大学, 大学院・へルスバイオサイエンス研究部, 准教授 (10396864)
• Project Period (FY): 2012
• Keywords: リサーチバイオリソース / ラット疾患モデル動物 / エクソーム / 次世代シーケンサー / 責任遺伝子変異 / ゲノム情報 / 連鎖解析

Research Abstract

In order to identify genes responsible for phenotypes observed in disease-model rats and determine molecular mechanisms producing those phenotypes, we modified our self-made rat exon capture system, which can easily analyze larger sets of exons in a liquid phase manner. We constructed an effective pipeline to analyze next-generation sequencing data with larger information of polymorphism and gene annotation data in rats. Through combining those systems with linkage analyses, we constructed effective and systematic tools for rat genome analysis. We finally applied this tool to a newly established rat model with unique phenotype, and validated usefulness of our system by collecting candidate variations possibly responsible for its phenotype.

Research Products

• [Journal Article] Esophageal squamous cell carcinoma developed 11 years after allogeneic bone marrow transplantation for acute lymphatic leukemia (2013)
  • Author(s): Miyawaki Y, Imoto I, Tokairin Y, Kawada K, Nakajima Y, Nishikage T, Nagai K, Kajiwara M, Inazawa J, Kawano T.
  • Journal Title: Jpn J Clin Oncol Volume: 43(1) Pages: 69-73
  • DOI: DOI:10.1093/jjco/hys184.
  • Peer Reviewed
• [Journal Article] DNA Methylation Signatures of Peripheral Leukocytes in Schizophrenia. (2013)
  • Author(s): Kinoshita M, Numata S, Tajima A, Shimodera S, Ono S, Imamura A, Iga JI, Watanabe S, Kikuchi K, Kubo H, Nakataki M, Sumitani S, Imoto I, Okazaki Y, Ohmori T
  • Journal Title: Neuromolecular Med Volume: 15(1)
  • DOI: DOI:10.1007/s12017-012-8198-6.
  • Peer Reviewed
• [Journal Article] Meta-analysis of association studies between DISC1 missense variants and schizophrenia in Japanese population (2012)
  • Author(s): Kinoshita M, Numata S, Tajima A, Ohi K, Hashimoto R, Shimodera S, Imoto I, Itakura M, Takeda M, Ohmori T.
  • Journal Title: Schizophr Res Volume: 141(2-3) Pages: 271-273
  • DOI: DOI:10.1038/onc.2011.646.
  • Peer Reviewed
• [Journal Article] Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor-suppressor in hepatocellular carcinoma (2012)
  • Author(s): Matsumura S, Imoto I, Kozaki K, Matsui T, Muramatsu T, Furuta M, Tanaka S, Sakamoto M, Arii S, Inazawa J.
  • Journal Title: Clin Cancer Res Volume: 18(13) Pages: 3541-3551
  • DOI: DOI:10.1158/1078-0432.CCR-11-1007.
  • Peer Reviewed
• [Journal Article] Genomic copy-number alterations of MYC and FHIT genes are associated with survival in esophageal squamous-cell carcinoma (2012)
  • Author(s): Miyawaki Y, Kawauchi H, Ooi A, Eishi Y, Kawano T, Inazawa J, Imoto I
  • Journal Title: Cancer Sci Volume: 103(8) Pages: 1558-1662
  • DOI: DOI:10.1111/j.1349-7006.2012.02329.x.
  • Peer Reviewed
• [Journal Article] The incidence of hypoplasia of the corpus callosum in patients with dup (X)(q28) involving MECP2 is associated with the location of distal breakpoints (2012)
  • Author(s): Honda S, Hayashi S, Nakane T, Imoto I, Kurosawa K, Mizuno S, Okamoto N, Kato M, Yoshihashi H, Kubota T, Nakagawa E, Goto YI, Inazawa J.
  • Journal Title: Am J Med Genet A Volume: 158A(6) Pages: 1292-1303
  • DOI: DOI:10.1002/ajmg.a.35321.
  • Peer Reviewed
• [Journal Article] SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation (2012)
  • Author(s): Ono H, Imoto I, Kozaki K, Tsuda H, Matsui T, Kurasawa Y, Muramatsu T, Sugihara K, Inazawa J.
  • Journal Title: Oncogene Volume: 31(47) Pages: 4923-4934
  • DOI: DOI:10.1038/onc.2011.646.
  • Peer Reviewed
• [Journal Article] Stabilization of phenotypic plasticity through mesenchymal-specific DNA hypermethylation in cancer cells. (2012)
  • Author(s): Kurasawa Y, Kozaki K, Pimkhaokham A, Muramatsu T, Ono H, Ishihara T, Uzawa N, Imoto I, Amagasa T, Inazawa J.
  • Journal Title: Oncogene Volume: 31(15) Pages: 1963-1974
  • DOI: DOI:10.1038/onc.2011.373.
  • Peer Reviewed
• [Presentation] Genetic variations in primary breast cancer and its metastatic tumor. (2012)
  • Author(s): Hosoda F, et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(札幌市)
  • Year and Date: 2012-09-21
• [Presentation] A tumor-suppressive microRNA cluster targets multiple cell cycle regulators in hepatocellular carcinoma. (2012)
  • Author(s): Furuta M,et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: さっぽろ芸文館(札幌市)
  • Year and Date: 2012-09-20
• [Presentation] Genomic copy-number alterations of MYC and FHIT genes are associated with survival in esophageal squamous-cell carcinoma (2012)
  • Author(s): Miyawaki Y, et al
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館(札幌市)
  • Year and Date: 2012-09-20
• [Presentation] Copy number alterations in urothelial carcinomas: Their clinicopathological correlation with DNA methylation. (2012)
  • Author(s): Nishiyama N, et al
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(札幌市)
  • Year and Date: 2012-09-20
• [Remarks] 徳島大学・大学院へルスバイオサイエンス研究部・人類遺伝学
  • URL: http://www.tokushima-u.ac.jp/med/culture/jinruiiden/