2013 Fiscal Year Final Research Report

Destruction of the multidrug efflux transporter on the plasma membrane by photodynamic treatment

Research Project

Project/Area Number	24650268
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Biomedical engineering/Biological material science
Research Institution	Saitama Medical University
Principal Investigator	MIYAMOTO YUICHI 埼玉医科大学, 保健医療学部, 准教授 (00313718)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	生物・生体工学 / ナノバイオ / 細胞・組織 / 光線力学的治療
Research Abstract	Photodynamic therapy (PDT) established as a curative treatment for the cancer has acquired anticancer efficacy by producing reactive oxygen species on the plasma membrane or the cytoplasmic organelle. In this study, I employed PDT inducing no remarkable cell injury and investigate whether the PDT can reduce the degree of the resistance of cisplatin- or paclitaxel-resistant HeLa cells. As the results, the cytotoxicity of paclitaxel for the paclitaxel-resistant HeLa cells was unchanged, whereas, the cytotoxicity of cisplatin for the cisplatin-resistant HeLa cells seems to increase. These findings suggest that the PDT is effective item for the reducing resistance of the anticancer drug- resistant cells possessing the cytoplasmic detoxification mechanism such as Glutathione(GSH) or metallothionein(MT) detoxification pathway.