Construction of a tissue-specific transcription factor-tethered extracellular matrix protein

2014 Fiscal Year Final Research Report

• Project/Area Number: 24650276
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: MIE Masayasu 東京工業大学, 総合理工学研究科(研究院), 准教授 (40334528)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 転写因子 / タンパク質導入 / バイオマテリアル

Outline of Final Research Achievements

In this study, a novel strategy for the construction of biomaterials is introduced by tethering a tissue-specific transcription factor protein to an artificial extracellular matrix (ECM) protein. Here, oligodendrocyte and motor neuron specific transcription factor Olig2 was tethered to an artificial ECM protein via coiled-coil helix formation. The artificial ECM, is comprised of an elastin-like peptide as a structural unit, as well as the AG73 peptide sequence derived from laminin and the C3 peptide sequence which binds to neural cell adhesion molecules (NCAMs) derived from the synthetic peptide library, for cell adhesion and enhance neurite outgrowth. As a proof of concept, tethered Olig2 was internalized into mouse embryonic carcinoma P19 cells and the ability to induce neural differentiation was investigated.

Free Research Field

組織工学