2013 Fiscal Year Final Research Report
Exsome sequencing of world-class sprint athletes
| Project/Area Number |
24650414
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Sports science
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
FUKU Noriyuki 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (40392526)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Masashi 地方独立行政法人東京都家健康長寿医療センタ-, 東京都健康長寿医療センター研究所, 部長 (60155166)
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| Co-Investigator(Renkei-kenkyūsha) |
IHARA Kunio 名古屋大学, 遺伝子実験施設, 助教 (90223297)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 短距離選手 / 全エクソン塩基配列 / 遺伝子多型 |
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| Research Abstract |
The purpose of the present study to identify genetic variants associated with anaerobic performance, namely, elite Jamaican (African ancestry) and Japanese sprint athlete status, from analysis of entire exon sequence. Total DNA was isolated from oral mucosa (Jamaican) and saliva (Japanese) with a QIAamp DNA Blood Mini Kit and Oragene DNA Collection Kit, respectively. Entire exon sequencing was analyzed by use of SOLiD4. A lots of sequence errors were found in Jamaican sprinters because of using DNA samples from oral mucosa, but not in Japanese sprinters. Therefore, we excluded in Jamaican athletes from further analysis. In the Japanese cohort, frequencies of the 11 variants were higher Five variants of them cause amino acid replacement. on the other hands, frequencies of the 15 variants were lower in elite Japanese sprinters than controls. Further extensive studies are necessary to confirm or identify genetic variants associated with elite Japanese sprint athlete status.
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[Journal Article] ACTN3 R577X Genotype is Associated with Sprinting in Elite Japanese Athletes2014
Author(s)
Mikami E, Fuku N, Murakami H, Tsuchie H, Takahashi H, Ohiwa N, Tanaka H, Pitsiladis YP, Higuchi M, Miyachi M, Kawahara T, Tanaka M
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Journal Title
Int J Sports Med
Volume: 35
Pages: 172-177
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[Journal Article] Lucia The rs1333049 polymorphism on locus 9p21.3 and extreme longevity in Spanish and Japanese cohorts2014
Author(s)
Pinós T, Fuku N, Cámara Y, Arai Y, Abe Y, Rodríguez-Romo G, Garatachea N, Santos-Lozano A, Miro-Casas E, Ruiz-Meana M, Otaegui I, Murakami H, Miyachi M, Garcia-Dorado D, Hinohara K, Andreu AL, Kimura A, Hirose N
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Journal Title
Age (Dordr)
Volume: 36
Pages: 933-943
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[Journal Article] Lucia ApoE gene and exceptional longevity : Insights from three independent cohorts2014
Author(s)
Garatachea N, Emanuele E, Calero M, Fuku N, Arai Y, Abe Y, Murakami H, Miyachi M, Yvert T, Verde Z, Zea MA, Venturini L, Santiago C, Santos-Lozano A, Rodríguez-Romo G, Ricevuti G, Hirose N, Rábano A
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Journal Title
Exp Gerontol
Volume: 53
Pages: 16-23
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[Journal Article] Association of the formiminotransferase N-terminal sub-domain containing gene and thrombospondin, type 1, domain-containing 7A gene with the prevalence of vertebral fracture in 2427 consecutive autopsy cases2013
Author(s)
Zhou H, Mori S, Kou I, Fuku N, Naka Mieno M, Honma N, Arai T, Sawabe M, Tanaka M, Ikegawa S, Ito H
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Journal Title
J Hum Genet
Volume: 58
Pages: 109-112
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[Journal Article] Exome sequencing of senescence-accelerated mice (SAM) reveals deleterious mutations in degenerative disease-causing genes2013
Author(s)
Tanisawa K, Mikami E, Fuku N, Honda Y, Honda S, Ohsawa I, Ito M, Endo S, Ihara K, Ohno K, Kishimoto Y, Ishigami A, Maruyama N, Sawabe M, Iseki H, Okazaki Y, Hasegawa-Ishii S, Takei S, Shimada A, Hosokawa M, Mori M, Higuchi K, Takeda T, Higuchi M, Tanaka M
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Journal Title
BMC Genomics
Volume: 14
Pages: 248
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[Journal Article] Ploutarhos S, Montgomery HE, Bailey ME, Pitsiladis YP. Association analysis of ACE and ACTN3 in elite Caucasian and East Asian swimmers2013
Author(s)
Wang G, Mikami E, Chiu LL, DE Perini A, Deason M, Fuku N, Miyachi M, Kaneoka K, Murakami H, Tanaka M, Hsieh LL, Hsieh SS, Caporossi D, Pigozzi F, Hilley A, Lee R, Galloway SD, Gulbin J, Rogozkin VA, Ahmetov II, Yang N, North KN
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Journal Title
Med Sci Sports Exerc
Volume: 45
Pages: 892-900
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[Journal Article] Pitsiladis Y. Genomics of Elite Sporting Performance : What little We Know and Necessary Advances2013
Author(s)
Wang G, Padmanabhan S, Wolfarth B, Fuku N, Lucia A, Ahmetov II, Cieszczyk P, Collins M, Eynon N, Klissouras V, Williams A
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Journal Title
Adv Genet
Volume: 84
Pages: 123-149
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[Journal Article] Genetic testing in Sports and exercise2013
Author(s)
Pitsiladis YP, Wang G, Padmanabhan S, Wolfarth B, Fuku N, Lucia A, Ahmetov II, Cieszczyk P, Collins M, Eynon N, Williams A
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Journal Title
The Sport and Exercise Scientist
Volume: 38
Pages: 16-17
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[Presentation] Montgomery HE, Pitsiladis Y : ACTN3 and ACE genotypes in elite Caucasian and Japanese swimmers2012
Author(s)
Wnag G, Mikami E, de Perini A, Bailey ME, Caporpssi D, Pigozzi F, Fuku N, Miyachi M, Kaneoka K, Murakami H, Tanaka M, Deson M, Hilley A, Lee R, Ahmetov II, Rogozkin VA, Galloway SD, Gulbin J, Yang N, North KN
Organizer
International Convention on Science, Education & Medicine in Sports 2012
Place of Presentation
Glasgow
Year and Date
20120719-24
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[Presentation] Genetic ancestry and elite sprinting in groups of west African Descent2012
Author(s)
Deson M, Wnag G, Fuku N, Mikami E, Tanisawa K, Scott R, Irwin L, Irving R, Charton V, Morrison E, Austin K, Tladi D, Headley S, Kolkhorst F, Tanaka M, Pitsiladis Y
Organizer
International Convention on Science, Education & Medicine in Sports 2012
Place of Presentation
Glasgow
Year and Date
20120719-24
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[Presentation] Ankle range of motion, foot arch characteristics and foot strength of habitually barefoot versus shod children in rural Kenya2012
Author(s)
Okutoyi P, Aibast H, Adero W, Bargoria VK, Sigei T, Chemjor D, Fuku N, Konstable K, Ongaro N, Lieberman DE, Pitsliladis Y
Organizer
International Convention on Science, Education & Medicine in Sports 2012
Place of Presentation
Glasgow
Year and Date
20120719-24
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[Presentation] ACTN3 And ACE Genotypes In Elite Japanese Track And Field Athletes2012
Author(s)
Eri Mikami, Noriyuki Fuku, Haruka Murakami, Hideyuki Takahashi, Nao Ohiwa, Yannis P. Pitsiladis, Hiroyasu Tsuchie, Mitsuru Higuchi, Motohiko Miyachi, Takashi Kawahara, Masashi Tanaka
Organizer
American College of Sports Medicine 59th Annual Meeting and 3rd World Congress on Exercise is Medicine
Place of Presentation
San Francisco
Year and Date
20120529-0602
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