2013 Fiscal Year Final Research Report

Phosphoinositide in the Golgi apparatus regulates epithelial-mesenchymal transition

Research Project

Project/Area Number	24650621
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Tumor biology
Research Institution	Kobe University
Principal Investigator	TOKUDA Emi (徳田恵美) 神戸大学, 医学(系)研究科(研究院), 医学研究員 (30598925)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	上皮間葉転換 / ゴルジ体 / イノシトールリン脂質
Research Abstract	In this study, I showed that phosphatidylinositol 4-phosphate (PI4P) in the Golgi apparatus regulates epithelial-mesenchymal transition during cancer progression. Increasing PI4P level in the Golgi apparatus resulted in decreased cell-cell adhesion and increased cell migration which is characteristic of epithelial-mesenchymal transition. In contrast, decreasing PI4P in the Golgi apparatus resulted in increased cell-cell adhesion and decreased invasion. Furthermore, PI4P level in the Golgi apparatus was increased in malignant breast cancer cell lines and malignant breast cancer patient. I also showed that GOLPH3, which is known to localize in the Golgi apparatus and bind to PI4P, was involved in the generation of these phenotypes in a manner that depends on its PI(4)P-binding ability. These results suggest that PI4P in the Golgi apparatus plays an important role in cancer progression.