2013 Fiscal Year Final Research Report
Development of novel cancer specific DDS utilizing circulating microRNA-derived mechanism
| Project/Area Number |
24650645
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical oncology
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| Research Institution | Hiroshima University |
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Principal Investigator |
TAHARA Hidetoshi 広島大学, 大学院医歯薬保健学研究院, 教授 (00271065)
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| Co-Investigator(Kenkyū-buntansha) |
OCHIYA Takahiro 国立がん研究センター研究所, 分子細胞治 療研究分野, 分野長 (60192530)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | がん / マイクロRNA / 核酸医薬 / ドラッグデリバリー |
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| Research Abstract |
We found that macrophage is highly secreted exosome like particle with exosome maker such as CD63 and CD9 and Alix. We made CD63-GFP fusion protein and cloned into lenti-virus vector. CD63-GFP lenti-virus is infected to cancer and macrophage and established cell line stably expressing CD63-GFP fusion protein. We isolated exosome from these cell lines and purified by ultra-centrifugation, and found that these purified exosome is successfully derived to recipient cells. These results suggest that exosome can be a tools for novel DDS of microRNAs.
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Research Products
(23 results)
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[Journal Article] Human telomerase reverse transcriptase and glucose-regulated protein 78 increase the life span of articular chondrocytes and their repair potential2012
Author(s)
Sato, M., Shin-ya, K., Lee, J.I., Ishihara, M., Nagai, T., Kaneshiro, N., Mitani, G., Tahara, H. & Mochida, J.
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Journal Title
BMC Musculoskelet Disorders
Volume: 13
Pages: 1-10
Peer Reviewed
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[Journal Article] Mutations in UVSSA cause UV-sensitive syndrome and destabilize ERCC6 in transcription-coupled DNA repair2012
Author(s)
Zhang X, Horibata K, Saijo M, Ishigami C, Ukai A, Kanno S, Tahara H, Neilan EG, Honma M, Nohmi T, Yasui A, Tanaka K
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Journal Title
Nature Genetics
Volume: 44
Pages: 593-597
Peer Reviewed
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