Roles of 53BP1 in elimination of an apoptotic cell

2013 Fiscal Year Final Research Report

• Project/Area Number: 24651056
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kanazawa Medical University
• Principal Investigator: IWABUCHI Kuniyoshi 金沢医科大学, 医学部, 教授 (10232696)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Akira 金沢医科大学, 医学部, 教授 (20344723) ; SUNATANI Yumi 金沢医科大学, 医学部, 助教 (70581057)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: アポトーシス

Research Abstract

p53 binding protein-1(53BP1) accumulates at sites of DNA double-strand breaks, where it suppresses DNA end resection, and facilitates repair of DNA double-strand breaks by non-homologous end joining. In this study, we found that in apoptotic cells 53BP1 is cleaved to generate a fragment of 53BP1 in a caspase-dependent manner. This 53BP1 fragment is localized on the surface of apoptotic cells. Our data suggest that 53BP1 regulates clearance of apoptotic cells by a macrophage.

Research Products

• [Journal Article] DHX36 enhances RIG-I signaling by facilitating PKR-mediated antiviral stress granule formation (2014)
  • Author(s): Yoo JS, Takahasi K, Ng CS, Ouda R, Onomoto K, Yoneyama M, Lai JC, Lattmann S, Nagamine Y, Matsui T, Iwabuchi K, Kato H, Fujita T
  • Journal Title: PLoS Pathog Volume: 10(3) Pages: e1004012
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• [Journal Article] RNA binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells (2014)
  • Author(s): Ishigaki Y, Nakamura Y, Tatsuno T, Hashimoto M, Iwabuchi K, Tomosugi N
  • Journal Title: Histochem Cell Biol Volume: 141(1) Pages: 101-109
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• [Journal Article] DNA Ligase IV and Artemis act cooperatively to suppress homologous recombination in human cells : Implications for DNA double-strand break repair (2013)
  • Author(s): Kurosawa A, Saito S, So S, Hashimoto M, Iwabuchi K, Watabe H, Adachi N
  • Journal Title: PLoS One Volume: 8 Pages: e72253
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• [Journal Article] Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human cells (2013)
  • Author(s): Ishigaki Y, Nakamura Y, Tatsuno T, Hashimoto M, Shimasaki T, Iwabuchi K, Tomosugi N
  • Journal Title: Exp Biol Med Volume: 238(8) Pages: 889-897
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• [Journal Article] Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer usingγ-H2AX foci of DNA damage in lymphocytes (2013)
  • Author(s): Doai M, Watanabe N, Takahashi T, Taniguchi M, Tonami H, Iwabuchi K, Kayano D, Fukuoka M, Kinuya S
  • Journal Title: Ann Nucl Med Volume: 27 Pages: 233-238
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• [Journal Article] Knockdown of stromal interaction molecule 1 (STIM1) suppresses store-operated calcium entry, cell proliferation and tumorigenicity in human epidermoid carcinoma A431 cells (2012)
  • Author(s): Yoshida J, Iwabuchi K, Matsui T, Ishibashi T, Masuoka T, Nishio M
  • Journal Title: Biochem Pharmacol Volume: 84 Pages: 1592-1603
  • Peer Reviewed
• [Presentation] 53BP1によるp53機能の制御 (2013)
  • Author(s): 松井理、砂谷優実、逆井良、橋本光正、岩淵邦芳
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  • Place of Presentation: 青森
  • Year and Date: 20131018-20
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• [Presentation] Calcium sensor STIM1 plays an essential role in human epidermoid carcinoma cell growth, migration, and tumorigenicity (2013)
  • Author(s): Yoshida J, Iwabuchi K
  • Organizer: 第72回日本癌学会学術総会
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  • Year and Date: 20131003-05
• [Presentation] Scilencing of calcium sensor protein stromal interoctionmolecule 1 (STIM1) suppresses cell proliferation and tumorigenicity in human epidermoid carcinoma A431 cells (2013)
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  • Organizer: 第86回日本薬理学会年会
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• [Presentation] Regulation of p21-mediated G1/S Checkpoint by 53BP1 (2012)
  • Author(s): Matsui T, Sunatani Y, Hashimoto M, Iwabuchi K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] Mechanism of Apoptosis Induction via Regulation of ASAP Complex by XRCC4 (2012)
  • Author(s): Sunatani Y, Kamdar R P, Sharma M K, Matsui T, Hashimoto M, Matsumoto Y, Iwabuchi K
  • Organizer: 第35回日本分子生物学会年会
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• [Presentation] Silencing of STIM1 suppresses store operated calcium entry and cell growth of human epidermoid carcinoma A431 cells (2012)
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  • Organizer: 第71回日本癌学会学術総会
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• [Presentation] テロメア末端融合における53BP1とRad18の機能解析 (2012)
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  • Organizer: 日本放射線影響学会第55回大会
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  • Year and Date: 20120906-08
• [Presentation] 53BP1によるp53機能の制御 (2012)
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• [Presentation] アポトーシスで生じたXRCC4N末断片によるDNA非損傷性アポトーシスの制御機構 (2012)
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• [Presentation] 低線量率放射線照射下におけるDNA2重鎖切断修復因子の役割 (2012)
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  • Organizer: 第48回金沢医科大学医学会学術集会
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  • Year and Date: 2012-07-21