2013 Fiscal Year Final Research Report
Development of fluorogenic probe for gene-analisis utilizing novel sililated fluorescent material
| Project/Area Number |
24651255
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオプローブ / 含ケイ素高輝度蛍光剤 |
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| Research Abstract |
Novel pyrene derivatives having modifiable silylated functional group were developed. Among these, a derivative possessing azide function was successfully incorporated into a modified DNA having alkynyl function via click chemistry. Also, a silylated pyrene possessing strong electron-withdrawing group (cyano function) was synthesized first time. The derivative exhibited marked red-shift on both absorption and emission spectra compare to that of the unmodified pyrene. In addition, fluorescent quantum yield of the derivative was increased almost twice as much as that of the unmodified pyrene. This unique derivative was further derived to the corresponding phosphoramide derivative and was incorporated into 5'-terminus of oligoDNA.
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