Development of method for the determination of thiol-containing metabolites using quantitative mass spectrometry

2014 Fiscal Year Final Research Report

• Project/Area Number: 24651265
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Keio University
• Principal Investigator: HISHIKI TAKAKO 慶應義塾大学, 医学部, 講師 (10338022)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ガスシグナル分子 / チオール化合物 / 硫化水素

Outline of Final Research Achievements

Hydrogen sulfide (H2S), physiological gasseous mediator, plays important roles in regulation of the vascular tone, cytoprotection, and long-term potentiation in hippocampus. In this study, we developed sensitive and quantitative analysis method for H2S and other thiol-containing metabolites, e.g., Cysteine, Cysteine persulfide (Cys-SH), Homocysteine(Hcy), Homocysteine persulfide (Hcy-SH), reduced-glutathione, reduced-glutathione persulfide (GSSH) with mass spectrometric technologies. We tried to quantify the thiol-containing molecules in the hearts of sulfide quinone reductase-like knockdown (SQR-KD) mice using this method. SQR has been identified to be responsible for the initial oxidation of sulfide in mitochondrial matrix. We could detect the higher level of thiol-containing metabolites (H2S, Cysteine persulfide, reduced-glutathione persulfide, and Homocysteine persulfide) in the SQR-KD mice hearts compared with control mice using this useful method.

Free Research Field

代謝生化学