Dynamics elucidation of zooxanthellae in scleractinian coral by comprehensive genetic analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24651267
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Resource conservation science
• Research Institution: Kochi University
• Principal Investigator: KUBOTA SATOSHI 高知大学, 教育研究部総合科学系, 准教授 (00314980)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 褐虫藻 / クレード / 網羅的解析 / 次世代シーケンサー

Outline of Final Research Achievements

The purpose of this study is to know the availability of next generation sequencer (NGS) for elucidation of zooxanthellae composition in scleractinian coral. Ribosomal RNA sequences of 28S region (LSU) was amplified and RFLP analysis and NGS analysis were applied. RFLP analysis showed several types of clade C and one clade F zooxanthellae in coral DNA. NGS analysis indicated major Clade C zooxanthellae species had identical sequences with analyzed by RFLP. This result supports that NGS must be powerful tool for elucidation of zooxanthella composition in scleractinian coral.

Free Research Field

水族生化学