How does dolphin detoxify mercury?

2013 Fiscal Year Final Research Report

• Project/Area Number: 24655065
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Ehime University
• Principal Investigator: AGUSA Tetsuro 愛媛大学, 沿岸環境科学研究センター, 特任助教 (50403853)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 水銀 / セレン / 解毒作用 / 海棲高等動物 / 環境化学 / 環境毒性学 / 分析化学 / メタロミクス

Research Abstract

Higher trophic marine animals accumulate a large amount of Hg in the liver. Most of Hg exists as tiemannite (HgSe) in those mammals. This compound is assumed to be an inert end product of the Hg detoxification process. However, it is still unknown the mechanism of HgSe formation. To understand the detoxification process of Hg, we investigated Hg binding proteins in the hepatic cytosol of the higher trophic marine animals.
By the purification of proteins interacted with Hg using an anion exchange and a gel filtration columns and an inductively coupled plasma - mass spectrometer (ICP-MS), four proteins with the molecular weight around 100 kDa and 40 kDa were obtained in the cytosol fraction of the northern fur seal (Callorhinus ursinus). The purified proteins may play a significant role in the formation of Hg-Se complex in the hepatic cytosol.

Research Products

• [Journal Article] Sedimentary records of metal depositions in Japanese alpine lakes for the last 250 years : Recent enrichments of airborne Sb and In in East Asia (2013)
  • Author(s): Kuwae, M., Tsugeki, N.K., Agusa, T., Toyoda, K., Yukinori, T., Ueda, S., Tanabe, S., Urabe, J
  • Journal Title: Science of the Total Environment Volume: 442 Pages: 189-197
  • DOI: 10.1016/j.scitotenv.2012.10.037
• [Journal Article] Exposure assessment of lead to workers and children in the battery recycling craft village, Dong Mai, Vietnam (2013)
  • Author(s): Noguchi, T., Itai, T., Tue, N.M., Agusa, T., Ha, N.N., Horai, S., Trang, P.T.K., Viet, P.H., Takahashi, S., Tanabe, S
  • Journal Title: Journal of Material Cycles and Waste Management Volume: 16(1) Pages: 46-51
  • DOI: 10.1007/s10163-013-0159-0
• [Journal Article] Human exposure to arsenic from drinking water in Vietnam (2013)
  • Author(s): Agusa, T., Trang, P.T.K., Lan, V.M., Anh, D.H., Tanabe, S., Viet, P.H., Berg, M
  • Journal Title: Science of the Total Environment Volume: (accepted in 2013/10/13)
  • DOI: 10.1016/j.scitotenv.2013.10.039
• [Journal Article] Exposure, metabolism, and toxic effect of arsenic in residents from arsenic-contaminated groundwater areas of southeast Asia, In Kawaguchi, M.. Misaki, K., Sato, H., Yokokawa, T., Itai, T. , Nguyen, T. M., Ono, J., Tanabe S. (Eds.) (2012)
  • Author(s): Agusa, T., Kunito, T., Kubota, R., Tanabe, S., Iwata, H.
  • Journal Title: International Symposium on Advanced Studies by Young Scientists on Environmental Pollution and Ecotoxicology, TERRAPUB, Tokyo Pages: 125-132
• [Journal Article] Individual variations in arsenic metabolism in Vietnamese : the association with arsenic exposure and GSTP1 genetic polymorphism (2012)
  • Author(s): Agusa, T., Kunito, T., Tue, N.M., Lan, V.T.M., Fujihara, J., Takeshita, H., Minh, T.B., Trang, P.T.K., Takahashi, S., Viet, P.H., Tanabe, S., Iwata, H
  • Journal Title: Metallomics Volume: 4 (1) Pages: 91-100
  • DOI: 10.1039/C1MT00133G
• [Journal Article] Genetic variation of FUT2 in a Vietnamese population : identification of two novel Se enzyme-inactivating mutations (2012)
  • Author(s): Soejima, M., Fujimoto, R., Agusa, T., Iwata, H., Fujihara, J., Takeshita, H., Minh, T.B., Trang, P.T.K., Viet, P.H., Nakajima, T., Tanabe, S., Koda, Y
  • Journal Title: Transfusion Volume: 52/6 Pages: 1268-1275
  • DOI: 10.1111/j.1537-2995.2011.03485.x
• [Journal Article] Multi-trace element levels and arsenic speciation in urine of e-waste recycling workers from Agbogbloshie, Accra in Ghana (2012)
  • Author(s): Asante, K.A., Agusa, T., Biney, C.A., Agyekum, W.A., Bello, M., Otsuka, M., Itai, T., Takahashi, S., Tanabe, S
  • Journal Title: Science of the Total Environment Volume: 424 Pages: 63-73
  • DOI: 10.1016/j.scitotenv.2012.02.072
• [Journal Article] Impact of metals in surface matrices from formal and informal electronic-waste recycling around Metro Manila, the Philippines, and intra-Asian comparison (2012)
  • Author(s): Fujimori, T., Takigami, H., Agusa, T., Eguchi, A., Bekki, K., Yoshida, A., Terazono, A., Ballesteros Jr, F.C
  • Journal Title: Journal of Hazardous Materials Volume: 221-222 Pages: 139-146
  • DOI: 10.1016/j.jhazmat.2012.04.019
• [Journal Article] Vanadium accelerates horizontal transfer of tet(M) gene from marine Photobacterium to E. coli (2012)
  • Author(s): Suzuki, S., Kimura, M., Agusa, T., Rahman, H
  • Journal Title: FEMS Microbiology Letter Volume: 336(1) Pages: 52-56
  • DOI: 10.1111/j.1574-6968.2012.02653.x
• [Journal Article] Changes in trophic conditions of Japanese mountain lakes due to increasing amounts of Asian dust (2012)
  • Author(s): Tsugeki, N.K., Agusa, T., Ueda, S., Kuwae, M., Oda, H., Tanabe, S., Tani, Y., Toyoda, K., Wan-lin, W. Urabe. J
  • Journal Title: Ecological Research Volume: 27(6) Pages: 1041-1052
  • DOI: 10.1007/s11284-012-0984-y
• [Presentation] (2014) Characterization of mercury binding proteins in the liver of northern fur seals (Callorhinus ursinus) (2014)
  • Author(s): Agusa, T., Yasugi, S., Ikemoto, T., Kunito, T., Baba, N., Tanabe, S., Iwata, H
  • Organizer: The Society of Toxicology (SOT) 53rd Annual Meeting
  • Place of Presentation: Phoenix, Arizona, USA, Abstract 335
  • Year and Date: 20140323-27
• [Presentation] ベトナム人におけるAS3MT遺伝子の発現量とSNPsの関係 (2013)
  • Author(s): 阿草哲郎, 國頭恭, Nguyen Minh Tue, Vi Thi Mai Lan, Tu Binh Minh, Pham Thi Kim Trang, 藤原純子, 竹下治男, 高橋真, Pham Hung Viet, 田辺信介, 岩田久人
  • Organizer: 第19回ヒ素シンポジウム
  • Place of Presentation: 福岡, 講演要旨集, 62-63
  • Year and Date: 20131100
• [Presentation] バイカルアザラシの微量元素蓄積とその応答遺伝子の探索 (2013)
  • Author(s): 阿草哲郎, 平川周作, 渡邉泉, 池本徳孝, 宇高真行, 金恩英, Evgeny A. Petrov, Valeriy B. Batoev, 田辺信介, 岩田久人
  • Organizer: 第22回環境化学討論会
  • Place of Presentation: 府中, 講演要旨集(in CD), 1-1B-2-1
  • Year and Date: 20130700
• [Presentation] (2013) Identification of Quantitative Trait Loci (QTL) Associated with Inorganic Arsenic Metabolism using Recombinant Inbred Strains of Mice (2013)
  • Author(s): Agusa, T., Komori, H., Soga, Y., Nose, M., Mori, S., Kubota, R., Tanabe, S., Iwata, H
  • Organizer: The XIII International Congress of Toxicology (ICT 2013)
  • Place of Presentation: Seoul, South Korea, Abstract Book, 205
  • Year and Date: 20130630-0704
• [Presentation] 組換え近交系マウスを用いたヒ素感受性遺伝子座の同定 (2013)
  • Author(s): 阿草哲郎, 小森浩章, 曽我美子, 宮崎龍彦, 能勢眞人, 森士朗, 久保田領志, 田辺信介, 岩田久人
  • Organizer: 第24回日本微量元素学会
  • Place of Presentation: 吹田, プログラム・抄録集, 103
  • Year and Date: 20130600
• [Presentation] (2013) Associations of single nucleotide polymorphisms and haplotypes in arsenic [+3 oxidation state] methyltransferase (AS3MT) with arsenic metabolism : A case study in arsenic contaminated areas from Vietnam (2013)
  • Author(s): Agusa, T., Kunito, T., Tue, N.M., Lan, V.T.M., Minh, T.B., Trang, P.T.K., Fujihara, J., Takeshita, H., Takahashi, S., Viet, P.H., Tanabe, S., Iwata, H
  • Organizer: The Society of Toxicology (SOT) 52nd Annual Meeting
  • Place of Presentation: San Antonio, Texsas, USA, Abstract 318
  • Year and Date: 20130300
• [Presentation] (2013) Arsenic exposure and susceptibility in Vietnamese (2013)
  • Author(s): Agusa, T., Kunito, T., Inoue, S., Minh, T.B., Tue, N.M., Ha, N.N., Tu, N.P.C., Trang, P.T.K., Fujihara, J., Takeshita, H., Takahashi, S., Tuyen, B.C., Viet, P.H., Iwata, H., Tanabe, S
  • Organizer: NIES Arsenic Workshop 2013 ~Seeking ways to reduce the arsenic health hazards from the roxicological standpoint~
  • Place of Presentation: Tsukuba, Japan, Abstracts, p.12
  • Year and Date: 2013-12-10
  • Invited
• [Presentation] Occupational exposure to trace elements in e-waste recycling workers; case studies from the Philippines and India (2013)
  • Author(s): Agusa, T., Takigami, H., Eguchi, A., Fujimori, T., Bekki, K., Yoshida, A., Terazono, A., Ballesteros Jr., F.C., Ha, N.N., Ramu, K., Murata, S., Parthasaraty, P., Bulbule, K.A., Takahashi, S., Subramanian, A., Iwata, H., Tanabe, S
  • Organizer: Workshop for Knowledge Sharing on E-waste in Thailand (The 9th NIES Workshop on E-Waste)
  • Place of Presentation: Bangkok, Thailand(ページ番号無し)
  • Year and Date: 2013-01-31
  • Invited
• [Presentation] Arsenic, manganese and barium contamination in groundwater and their human exposure in Vietnam (2013)
  • Author(s): Agusa, T., Kunito, T., Inoue, S., Minh, T.B., Tue, N.M., Ha, N.N., Tu, N.P.C., Trang, P.T.K., Takahashi, S., Tuyen, B.C., Viet, P.H., Iwata, H., Tanabe, S
  • Organizer: Japan-Vietnam Joint Workshop on"Current Challenges for Managing Environmental Issues and Waste Recycling in Northern Vietnam"
  • Place of Presentation: Hanoi, Vietnam, Abstract book, 14
  • Year and Date: 2013-01-08
• [Presentation] (2012) Accumulation feature of trace elements in mass-stranded harbor seals (Phoca vitulina) in the North Sea coast in 2002 (2012)
  • Author(s): Agusa, T., Yasugi, S., Iida, A., Ikemoto, T., Anan, Y., Kuiken, T., Osterhaus, A.D.M.E., Tanabe, S., Iwata, H
  • Organizer: SETAC (Society of Environmental Toxicology and Chemistry) North America 33rd Annual Meeting
  • Place of Presentation: Long Beach, California, USA, Abstract Book, 207-208
  • Year and Date: 20121111-15
• [Presentation] アジア途上国における地下水のヒ素汚染とヒトへの影響 (2012)
  • Author(s): 阿草哲郎、岩田久人 ,國頭恭, 井上英, 久保田領志, Karri Ramu, Tu Binh Minh, Nguyen Minh Tue, Nguyen Ngoc Ha, Nguyen Phuc Cam Tu, 藤原純子, Paromita Charaborty, Chhoun Chamnan, Pham Thi Kim Trang, Alissara Reungsang, Kriengsak Srisuk, 中村哲, 滝沢智, 竹下治男, 高橋真, Annamalai Subramanian, Bui Cach Tuyen, Pham Hung Viet, Touch Seang Tana, 田辺信介
  • Organizer: 第18回ヒ素シンポジウム
  • Place of Presentation: 宮崎, 講演要旨集, 40-41
  • Year and Date: 20121100
• [Presentation] インドのe-wasteリサイクル労働者における微量元素曝露の実態 (2012)
  • Author(s): 阿草哲郎, Annamalai Subramanian, Peethambaram Parthasarathy, Keshav A. Bulbule,高橋真, 岩田久人, 田辺信介
  • Organizer: 第23回廃棄物資源循環学会研究発表会
  • Place of Presentation: 仙台, 研究発表会講演論文集, 589-590
  • Year and Date: 20121000
• [Presentation] ベトナム人の無機ヒ素メチル化能力とAS3MTのSNPsの関係 (2012)
  • Author(s): 阿草哲郎, 國頭恭, Nguyen Minh Tue, Vi Thi Mai Lan, Tu Binh Minh, Pham Thi Kim Trang, 高橋真, 藤原純子, 竹下治男, Pham Hung Viet、田辺信介、岩田久人
  • Organizer: 第23回日本微量元素学会
  • Place of Presentation: 東京都千代田区, 抄録集, 171
  • Year and Date: 20120700
• [Presentation] ヒ素代謝・排泄に関与する感受性遺伝子の探索 (2012)
  • Author(s): 阿草哲郎, 小森浩章, 曽我美子, 宮崎龍彦, 能勢眞人, 森士朗, 久保田領志, 田辺信介, 岩田久人
  • Organizer: 第21回環境化学討論会
  • Place of Presentation: 松山, 講演要旨集(in CD), 2D-15
  • Year and Date: 20120700
• [Presentation] インドおよびフィリピンのe-wasteリサイクル労働者における微量元素曝露の実態 (2012)
  • Author(s): 阿草哲郎, 滝上英孝, 藤森崇, 江口哲史, 戸次加奈江, 吉田綾, 寺園淳, Florencio C. Ballesteros Jr., Keshav A. Bulbule, Peethmbaram Parthasaraty, 高橋真, Annamalai Subramanian, 岩田久人, 田辺信介
  • Organizer: 第21回環境化学討論会
  • Place of Presentation: 松山, 講演要旨集(in CD), P-012
  • Year and Date: 20120700
• [Presentation] Associations of 18 single nucleotide polymorphisms in As [+3 oxidation state] methyltransferase (AS3MT) with As methylation capacity in Vietnamese (2012)
  • Author(s): Agusa, T., Kunito, T., Tue, N.M., Lan, V.T.M., Minh, T.B., Trang, P.T.K., Fujihara, J., Takeshita, H., Takahashi, S., Viet, P.H., Tanabe, S., Iwata, H
  • Organizer: The Ottawa 2012 International Symposium on Biochemistry & Biophysics
  • Place of Presentation: Ottawa, Canada, PROGRAM & ABSTRACT BOOK, 114-115
  • Year and Date: 2012-10-25
  • Invited
• [Presentation] 遺伝子レベルのヒ素代謝機構、人種間での感受性の差 (2012)
  • Author(s): 阿草哲郎
  • Organizer: 日本化学会中国四国支部広島地区化学講演会
  • Place of Presentation: 東広島
  • Year and Date: 2012-10-15
  • Invited
• [Remarks]
  • URL: http://ecotoxiwata.jp/
• [Remarks]
  • URL: http://researchmap.jp/read0097650/?lang=japanese