2013 Fiscal Year Final Research Report
Single molecule detection usign Biological/MOP nanopore system
| Project/Area Number |
24655072
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | Kanagawa Academy of Science and Technology |
|---|
Principal Investigator |
KAWANO Ryuji 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (90401702)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
FURUKAWA Shuhei 京都大学, 物質-細胞統合システム拠点, 准教授 (90452276)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 脂質二分子膜 / 膜タンパク質 / MEMS / 金属錯体 / 配位高分子 / 一分子計測 / MOP |
|---|
| Research Abstract |
This project proposes a strategy for single molecule detection using a synthetic metal-organic cage embedded into a channel protein. A single molecule detection based on biological nanopore, such as pore-forming proteins, has been studied as a high sensitive biosensor. However, the size of the biological nanopore is difficult to control because of their inherent structure. In this study, we synthesized metal-organic polyhedra which had ~ 0.6 nm nanopore as a nanocage, and we succesflly integrated this synthetic nanopore with an a-hemolysin (biological) nanopore.
|
