2013 Fiscal Year Final Research Report
Visualization of subcellular thermoregulation in live cells using fluorescent coiled-coil proteins
| Project/Area Number |
24655151
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | サーモセンサー / 蛍光タンパク質 / coiled-coil / tsGFP |
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| Research Abstract |
In mammals and birds, thermoregulation to conserve body temperature is vital to life. Multiple mechanisms of thermogeneration localized in different subcellular organelles have been proposed. However, previous studies have failed to observe thermogenesis directly in intact organelles. Here, we have visualized intracellular thermogenesis by using a fluorescent thermosensor, tsGFP. Specific targeting of tsGFPs enables visualization of thermogenesis in discrete organelles, notably mitochondria in brown adipocytes and the endoplasmic reticulum in myotubes. Strikingly, in HeLa cells, targeted tsGFP reveals heterogeneity in mitochondrial thermogenesis that is well correlated with the electrochemical gradient. Thus, tsGFPs directly demonstrate subcellular thermotransduction, and will serve as powerful tools to assess thermogenesis in vivo.
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[Journal Article] Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells2013
Author(s)
Kiyonaka S, Kajimoto T, Sakaguchi R, Shinmi D, Omatsu-Kanbe M, Matsuura H, Imamura H, Yoshizaki T, Hamachi I, Morii T, Mori Y
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Journal Title
Nat. Methods
Volume: 10
Pages: 1232-1238
DOI
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[Journal Article] Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy2012
Author(s)
Tadmouri A, Kiyonaka S, Barbado M, Rousset M, Fablet K, Sawamura S, Bahembera E, PErnet-Gallay K, Arnoult C, Miki T, Sadoul K, Gory-Faure S, Lambrecht C, Lesage F, Akiyama S, Khochbin S, Baulande S, Janssens V, Andrieux A, Dolmetsch R, Ronjat M, Mori Y, De Waard M
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Journal Title
EMBO J
Volume: 31
Pages: 3730-3744
DOI
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