2013 Fiscal Year Final Research Report
Investigation of rheological effect on Hepatocyte isolation procedure for liver regeneration
| Project/Area Number |
24656129
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Fluid engineering
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OBARA Hiromichi 首都大学東京, 大学院理工学研究科, 助教 (80305424)
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| Co-Investigator(Renkei-kenkyūsha) |
ENOSAWA Shin 独立行政法人国立成育医療研究センター, 研究所先端医療開発室, 室長 (40232962)
MATSUNO Naoto 独立行政法人国立成育医療研究センター, 研究所先端医療開発室, 特別研究員 (00231598)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ﾊﾞｲｵ流体力学 / 細胞治療 / 移植 / せん断 / マイクロチャネル |
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| Research Abstract |
Hepatocyte isolation procedure using a perfusion is one of the most important process for improvements of cell transplantation, developments of an artificial biological liver and establishments of a liver regeneration. In this study, three types of experiments were employed to investigate the rheological effect of the flow on the hepatocyte viabilities and functions during the isolation procedures. The micro-channel experiment imitated the clinical procedures using a needle and catheters was employed to clarify the effect of the shear stress induced near wall region in micro-channel. The rotating disks experiment was employed to investigate the effect of the shear stress intensity and exposure time on the cell viability. Furthermore, the evaluation experiment for the cell function was conducted to find the difference of the metabolisms of ammonia between isolated single cells and clustered cells.
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