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2014 Fiscal Year Final Research Report

Study on high performance hydrogen-methane fermentation process

Research Project

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Project/Area Number 24656314
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Civil and environmental engineering
Research InstitutionPrefectural University of Hiroshima

Principal Investigator

KAZUYUKI Nishimura  県立広島大学, 生命環境学部, 教授 (00261595)

Project Period (FY) 2012-04-01 – 2015-03-31
Keywords水素発酵 / メタン発酵 / リアルタイムPCR / mcrA gene
Outline of Final Research Achievements

In order to produce an efficient hydrogen-methane fermentation process, the behavior of microorganisms in a fermenter was examined by the activity of the gene.When hydrogen fermentation threw a raw material into a reactor, it was indicated that it's important to revise pH around 7.5.The hydrogen-methane fermented process needs one compared with the capacity of each reactor about 10 times.The determination method of mcrA gene created the primer set from the result of microflora or reference, and was examined by the real-time PCR method.The determination method of the amount of mcrA gene by the real-time PCR method has been established when the primer set of mcrA gene for Methanoculles obtained from reference was used.The expression level of mcrA gene in the continuous methane fermentation experiment system was quantified by real-time RT-PCR method which used the primer set of mcrA gene for Methanoculles.

Free Research Field

環境衛生工学

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Published: 2016-06-03  

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