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2014 Fiscal Year Final Research Report

Real-time monitoring of protein levels

Research Project

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Project/Area Number 24657002
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genetics/Genome dynamics
Research InstitutionNagoya University

Principal Investigator

ISHIURA Masahiro  名古屋大学, 遺伝子実験施設, 研究員 (20132730)

Co-Investigator(Kenkyū-buntansha) MATSUO Takuya  名古屋大学, 遺伝子実験施設, 助教 (00452201)
Project Period (FY) 2012-04-01 – 2015-03-31
Keywords生物時計 / リアルタイム測定
Outline of Final Research Achievements

We tryed to develop a monitoring system for protein levels in vivo in real time and to apply the system to studies for the circadian clock. For this purpose, we used clock proteins of the green alga Chlamydomonas as a model. As a result, we succeeded in monitoring of levels in ROC15, ROC40, ROC66, and ROC75 Chlamydomonas clock proteins in vivo in real time. In addition, we succeeded in analyzing properties of the clock proteins by using this system.

Free Research Field

生物時計

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Published: 2016-06-03  

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