2013 Fiscal Year Final Research Report
Identification of genes for enzymes involved in betalain biosynthesis and expression analysis in non-Dianthus plants.
| Project/Area Number |
24657026
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OZEKI Yoshihiro 東京農工大学, 工学(系)研究科(研究院), 教授 (50185592)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ベタレイン / オシロイバナ / Mirabilis jalapa / L-DOPA / cyclo-DOPA / CYP76AD3 / トランスポゾン |
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| Research Abstract |
In order to elucidate the unidentified enzymes including betalain synthesis, the sepals of four o'clock were analyzed by RNA-seq. Nine candidate cDNAs for the enzyme catalyzing tyrosine to L-DOPA, which was the first key step enzyme of betalain synthesis, were identified. The genomic structure of one of them, CYP76AD3, which was homolog of CYP76AD1 derived from red beet, was revealed. In the CYP76AD3 gene of variegated sepals with red sectors or dots on a yellow background, CACTA transposable element, dTmj1, was inserted in the intron of CYP76AD3 gene to disturb its transcription, resulting to prevent the expression of CYP76AD3 activity or red betacyanin synthesis, but to synthesize yellow betaxanthins. The movement of dTmj1 in this variegated sepals caused to revert the gene activity and the synthesis of betacyanin revived to generate red sectors or dots on a yellow background.
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Research Products
(6 results)
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[Journal Article] Genetic engineering of yellow betalain pigments beyond the species barrier2013
Author(s)
Nakatsuka, T., Yamada, E., Takahashi, H., Imamura, T., Suzuki, M., Ozeki, Y., Tsujimura, I., Saito, M., Sakamoto, Y., Sasaki, N. and Nishihara, M.
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Journal Title
Scientific Reports
Volume: Vol. 3
Pages: 1970
DOI
Peer Reviewed
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