2013 Fiscal Year Final Research Report
Experimental demonstration of functional compensation among paralogous genes encoding neuropeptides
| Project/Area Number |
24657050
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OKA Yoshitaka 東京大学, 理学(系)研究科(研究院), 教授 (70143360)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ペプチド / 生殖 / 性行動 / パラログ遺伝子 / トランスジェニック / メダカ |
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| Research Abstract |
There is a general agreement that a whole genome duplication has occurred early during evolution to produce paralogous genes with diversified functions. Functional compensation of a deleted gene by its paralogous gene has been documented for unicellular organisms, but such functional compensation has not been demonstrated for multicellular organisms so far. We have recently found an example of possible functional compensation between the paralogous genes, kiss1 and kiss2, encoding peptides called kisspeptins, which are considered to be involved in the central regulation of reproduction and/or sexual behavior. Here, we attempted at demonstrating experimentally functional compensation mechanisms of kisspeptin genes using state-of-the-art gene knock-out techniques such as TALEN and CRISPR/Cas9, which can be applied to non-model organisms.
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[Journal Article] Time-of-day dependent changes in GnRH1 neuronal activities and gonadotropin mRNA expression in a daily spawning fish, medaka2012
Author(s)
Karigo, T., Kanda, S., Abe, H., Okubo, K., and Oka, Y.
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Journal Title
Endocrinology
Volume: 153
Pages: 3394-3404
DOI
Peer Reviewed
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[Journal Article] Female-specific target sites for both estrogen and androgen in the teleost brain2012
Author(s)
Hiraki, T., Takeuchi, A., Tsumaki, T., Zempo, B., Kanda, S., Oka, Y., Nagahama, Y., and Okubo, K.
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Journal Title
Proceedings of the Royal Society. B, Biological sciences
Volume: 279
Pages: 5014-5023
DOI
Peer Reviewed
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[Presentation] Morphological analysis of the early development of GnRH neuron systems in the EGFP-expressing transgenic medaka lines2013
Author(s)
Takahashi, A., Islam, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 43th Annual Meeting of the Society for Neuroscience 2013
Place of Presentation
San Diego
Year and Date
20131109-13
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[Presentation] Embryonic Expression and Function of Kisspeptins and their Receptors in a Teleost Fish, medaka2012
Author(s)
Okubo, K., Hodne, K., Kanda, S., Shimada, H., Oka, Y., Weltzien, F.A.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo
Year and Date
20121106-09
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[Presentation] Electrophysiological and Morphological Analysis of Kiss1 Neurons in Trasgenic Medaka, Oryzias latipes2012
Author(s)
Shimada, H., Kanda, S., Akazome, Y., Abe, H., Okubo, K., Oka, Y.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo
Year and Date
20121106-09
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[Presentation] Effects of Kisspeptins on LH Secretion and GnRH1 Neuronal Activities in Goldfish and Medaka2012
Author(s)
Karigo, T., Uenoyama, Y., Oishi, S., Fujii, N., Mitani, Y., Kanda, S., Oka, Y.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo
Year and Date
20121106-09
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